We bring you the latest news from the healthcare about the health care in the United Kingdom.

vrijdag 14 september 2018

Nature Protocols Contents: Volume 13 Number 9

If you are unable to see the message below, click here to view.
Nature Protocols

September 2018 Volume 13, Issue 9

Do you have a career question?

The Naturejobs podcast features one-on-one Q&As, panel discussions and other exclusive content to help scientists with their careers. Hosted on the Naturejobs blog, the podcast is also available on iTunes and Soundcloud. 

Listen today!




High-throughput and high-sensitivity phosphoproteomics with the EasyPhos platform    pp1897 - 1916
Sean J. Humphrey, Ozge Karayel, David E. James & Matthias Mann

This protocol describes the EasyPhos platform, a high-throughput and user-friendly workflow for reliable identification of phosphopeptides from small amounts (<200 μg) of sample.


A three-dimensional engineered heterogeneous tumor model for assessing cellular environment and response    pp1917 - 1957
Darren Rodenhizer, Teresa Dean, Bin Xu, Dan Cojocari & Alison P. McGuigan

This protocol describes TRACER, a 3D cell culture system that enables the assembly of heterogeneous model tumors or tissues that easily disassemble for rapid analysis of different cell populations from particular microenvironments.


PhIP-Seq characterization of serum antibodies using oligonucleotide-encoded peptidomes    pp1958 - 1978
Divya Mohan, Daniel L. Wansley, Brandon M. Sie, Muhammad S. Noon, Alan N. Baer et al.

Phage immunoprecipitation sequencing (PhIP-Seq) is a method for analyzing antibody-repertoire binding specificities. Phage-displayed oligonucleotide libraries encoding peptidomes are immunoprecipitated and analyzed by high-throughput DNA-Seq.


Nature Protocols
JOBS of the week
Postdoctoral Researcher (f / m)
German Center for Neurodegenerative Diseases (DZNE)
Faculty Positions Department of Cell and Molecular Biology
St. Jude Children's Research Hospital (SJCRH)
Postdoctoral Fellow
University of Washington
Research Fellow
Mayo Clinic
Faculty Position in Innate Immunology
Mayo Clinic
More Science jobs from
Nature Protocols

More science events from

Fit-free analysis of fluorescence lifetime imaging data using the phasor approach    pp1979 - 2004
Suman Ranjit, Leonel Malacrida, David M. Jameson & Enrico Gratton

This protocol describes fit-free analysis of fluorescence lifetime imaging microscopy (FLIM) data using the phasor approach. Pixel-by-pixel decays are transformed to the phasor space, and then the clusters can be connected to the image by the reciprocity rules of the phasor plots.


Accessing crystal–crystal interaction forces with oriented nanocrystal atomic force microscopy probes    pp2005 - 2030
Xin Zhang, Yang He, Jia Liu, Mark E. Bowden, Libor Kovarik et al.

This protocol describes focused ion beam–milling approaches for fabricating oriented single-nanocrystal atomic force microscopy probes, as well as how to measure direction-specific interaction forces between nanocrystals in solution and in vacuum.


Configuration of electrical spinal cord stimulation through real-time processing of gait kinematics     pp2031 - 2061
Marco Capogrosso, Fabien B. Wagner, Jerome Gandar, Eduardo Martin Moraud, Nikolaus Wenger et al.

This protocol describes how to configure targeted spinal cord stimulation using electromyographic recordings and real-time processing of gait kinematics to enable voluntary control of specific leg movements in rats and nonhuman primates.


Generation and assembly of human brain region–specific three-dimensional cultures    pp2062 - 2085
Steven A. Sloan, Jimena Andersen, Anca M. Paşca, Fikri Birey & Sergiu P. Paşca

Neural differentiation and self-organization of hPSCs are induced by culture in suspension. Neural spheroids are then differentiated into dorsal or ventral forebrain spheroids that can be combined to obtain functionally integrated human assembloids.


Preparation of asymmetric phospholipid vesicles for use as cell membrane models    pp2086 - 2101
Milka Doktorova, Frederick A. Heberle, Barbara Eicher, Robert F. Standaert, John Katsaras et al.

Improved and robust procedures for a cyclodextrin-mediated preparation of asymmetric large unilamellar vesicles of diverse lipid compositions and the characterization of their degree of asymmetry and individual leaflet compositions with NMR and GC.


Searching for a new career? 

At Nature Research we frequently recruit talented individuals to join our editorial and publishing teams. If you have a PhD and a passion for science this may be the perfect career for you.

Visit our research editorial and publishing careers website to discover more about what we do.

PEG-4MAL hydrogels for human organoid generation, culture, and in vivo delivery    pp2102 - 2119
Ricardo Cruz-Acuña, Miguel Quirós, Sha Huang, Dorothée Siuda, Jason R. Spence et al.

This protocol describes how to use a fully defined, synthetic hydrogel to support the in vitro generation and culture of human organoids derived from pluripotent stem cells and the in vivo delivery of hydrogel-encapsulated organoids into mouse colon.


Nature Briefing is an essential round-up of science news, opinion and analysis, free in your inbox every weekday. With Nature Briefing, we'll keep you updated on the latest research, so you can focus on yours.

Click here to sign up.
nature events
Natureevents is a fully searchable, multi-disciplinary database designed to maximise exposure for events organisers. The contents of the Natureevents Directory are now live. The digital version is available here.
Find the latest scientific conferences, courses, meetings and symposia on natureevents.com. For event advertising opportunities across the Nature Publishing Group portfolio please contact natureevents@nature.com
More Nature Events

You have been sent this Table of Contents Alert because you have opted in to receive it. You can change or discontinue your e-mail alerts at any time, by modifying your preferences on your nature.com account at: www.nature.com/myaccount
(You will need to log in to be recognised as a nature.com registrant)

For further technical assistance, please contact our registration department

For print subscription enquiries, please contact our subscription department

For other enquiries, please contact our customer feedback department

Springer Nature | One New York Plaza, Suite 4500 | New York | NY 10004-1562 | USA

Springer Nature's worldwide offices:
London - Paris - Munich - New Delhi - Tokyo - Melbourne
San Diego - San Francisco - Washington - New York - Boston

Macmillan Publishers Limited is a company incorporated in England and Wales under company number 785998 and whose registered office is located at The Campus, 4 Crinan Street, London, N1 9XW.

Nature is part of Springer Nature. © 2018 Springer Nature Limited. All rights reserved.

Springer Nature