We bring you the latest news from the healthcare about the health care in the United Kingdom.

woensdag 8 augustus 2018

Nature Protocols Contents: Volume 13 Number 8

If you are unable to see the message below, click here to view.
Nature Protocols
TABLE OF CONTENTS

August 2018 Volume 13, Issue 8

Protocols
 
Advertisement
 
Do you have a career question?

The Naturejobs podcast features one-on-one Q&As, panel discussions and other exclusive content to help scientists with their careers. Hosted on the Naturejobs blog, the podcast is also available on iTunes and Soundcloud. 

Listen today!

 

Protocols

 

A general, electrocatalytic approach to the synthesis of vicinal diamines    pp1725 - 1743
Niankai Fu, Gregory S. Sauer & Song Lin
doi:10.1038/s41596-018-0010-0

1,2-diamines are commonly found in pharmaceutically relevant compounds. They can be prepared from diazides. This protocol describes an electrocatalytic method for preparing a wide range of diazides from alkenes, using a manganese catalyst.

 

Quantitative analysis of newly synthesized proteins    pp1744 - 1762
Yuanhui Ma, Daniel B. McClatchy, Salim Barkallah, William W. Wood & John R. Yates III
doi:10.1038/s41596-018-0012-y

This protocol describes a proteomics approach for quantifying newly synthesized proteins (NSPs). NSPs are pulse-labeled with the methionine analog AHA or a heavy-isotope version of it, followed by AHA-peptide enrichment and LC-MS/MS analysis.

 

Assessing spatial pattern separation in rodents using the object pattern separation task    pp1763 - 1792
Nick P. van Goethem, Britt T. J. van Hagen & Jos Prickaerts
doi:10.1038/s41596-018-0013-x

In this behavioral task, two objects are placed at specific locations at different times, and the response of mice and rats to these positions is assessed. The results indicate the rodents’ ability to recognize changes in spatial pattern separation.

 

Microfabrication of AngioChip, a biodegradable polymer scaffold with microfluidic vasculature    pp1793 - 1813
Boyang Zhang, Benjamin Fook Lun Lai, Ruoxiao Xie, Locke Davenport Huyer, Miles Montgomery et al.
doi:10.1038/s41596-018-0015-8

This protocol describes the fabrication of AngioChip, a microfabricated scaffold for engineering vascularized tissues. In addition, the protocol contains procedures for immunostaining, drug screening and surgical anastomosis of the AngioChip.

 

Pig lung transplant survival model    pp1814 - 1828
Andrea Mariscal, Lindsay Caldarone, Jussi Tikkanen, Daisuke Nakajima, Manyin Chen et al.
doi:10.1038/s41596-018-0019-4

This protocol describes a pig model in which lungs are transplanted into recipients who are subsequently monitored for 3 d after surgery to study ischemia reperfusion injury and early graft function.

 

Identification of RNA-binding protein targets with HyperTRIBE    pp1829 - 1849
Reazur Rahman, Weijin Xu, Hua Jin & Michael Rosbash
doi:10.1038/s41596-018-0020-y

HyperTRIBE uses a hyperactive RNA-editing enzyme fused to an RNA-binding protein (RBP) to mark the target RNA transcripts of the RBP by converting adenosine to inosine near the binding sites with increased efficiency and reduced sequence bias.

 

Noninvasive 40-Hz light flicker to recruit microglia and reduce amyloid beta load    pp1850 - 1868
Annabelle C. Singer, Anthony J. Martorell, J. Miller Douglas, Fatema Abdurrob, Matthew K. Attokaren et al.
doi:10.1038/s41596-018-0021-x

This protocol describes a noninvasive approach to evoke microglial engulfment and reduce amyloid levels in mouse brain. The authors describe assembly and operation of a light-flicker system, as well as assessment of the molecular and cellular effects.

 

Combined expansion microscopy with structured illumination microscopy for analyzing protein complexes    pp1869 - 1895
Yongfu Wang, Zulin Yu, Cori K. Cahoon, Tari Parmely, Nancy Thomas et al.
doi:10.1038/s41596-018-0023-8

This protocol describes how to combine expansion microscopy (ExM) with structured illumination microscopy (SIM). ExM–SIM is exemplified by super-resolution analysis of the synaptonemal complex (SC) and single-particle averaging of SC proteins.

 

Nature Protocols
JOBS of the week
Senior Research Scientist in Stem Cell Biology
University of Illinois - Chicago
Lab Manager Position
Institute of Molecular Biotechnology (IMBA)
Associate / Scientist, Candidate Transition (CMC Development)
Immunocore
IBMS Seeking Assistant / Associate / Full Professor
The Institute of Basic Medical Sciences (IBMS)
Associate Senior Lecturer
Swedish University of Agricultural Sciences (SLU)
More Science jobs from
Nature Protocols
EVENT

 
More science events from
Advertisement
Nature Briefing is an essential round-up of science news, opinion and analysis, free in your inbox every weekday. With Nature Briefing, we'll keep you updated on the latest research, so you can focus on yours.

Click here to sign up.
 
nature events
Natureevents is a fully searchable, multi-disciplinary database designed to maximise exposure for events organisers. The contents of the Natureevents Directory are now live. The digital version is available here.
Find the latest scientific conferences, courses, meetings and symposia on natureevents.com. For event advertising opportunities across the Nature Publishing Group portfolio please contact natureevents@nature.com
More Nature Events

You have been sent this Table of Contents Alert because you have opted in to receive it. You can change or discontinue your e-mail alerts at any time, by modifying your preferences on your nature.com account at: www.nature.com/myaccount
(You will need to log in to be recognised as a nature.com registrant)

For further technical assistance, please contact our registration department

For print subscription enquiries, please contact our subscription department

For other enquiries, please contact our customer feedback department

Springer Nature | One New York Plaza, Suite 4500 | New York | NY 10004-1562 | USA

Springer Nature's worldwide offices:
London - Paris - Munich - New Delhi - Tokyo - Melbourne
San Diego - San Francisco - Washington - New York - Boston

Macmillan Publishers Limited is a company incorporated in England and Wales under company number 785998 and whose registered office is located at The Campus, 4 Crinan Street, London, N1 9XW.

Nature is part of Springer Nature. © 2018 Springer Nature Limited. All rights reserved.

Springer Nature