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donderdag 17 mei 2018

Nature Methods Application Notes e-UPDATE: 17 May 2018

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17 May 2018 
Application Notes bring you the latest information about innovative tools and technologies and their applications in the lab. We hope that you will find this service useful and informative and encourage you to sign up for future Updates to ensure that you never miss one!
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FEATURED APPLICATION NOTE
TwistAmp® Liquid: a versatile amplification method to replace PCR
www.twistdx.co.uk/en/rpa >
Here we introduce TwistAmp® Liquid, a new PCR replacement format that makes RPA technology more amenable to a wide range of research applications. In contrast to PCR amplification, RPA takes minutes, rather than hours, and can be run with little to no equipment. TwistAmp® Liquid Basic and Basic RT kits can be used for applications requiring good fidelity, gel electrophoresis or solid phase detection. TwistAmp® Liquid exo and exo RT allow rapid real-time amplification and detection of targets without sacrificing sensitivity or specificity.
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Recovering droplets from Sapphire Chips
www.stillatechnologies.com >
The Naica System allows the recovery of droplets from the Sapphire chips with a simple procedure that can be performed before or after PCR.
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Controlling the topography and biochemistry of cell culture substrates with PRIMO® photopatterning system
www.alveolelab.com >
In vivo, the cellular microenvironment has a crucial impact on the regulation of cell behavior and functions such as cellular differentiation, proliferation and migration. One of the challenges confronting cell biologists is to mimic this microenvironment in vivo to more efficiently study living cells and model diseases. Here we present PRIMO: a contactless and maskless UV projection system, which allows to control the topography and chemistry of in vivo microenvironments. We first show that PRIMO is a suitable tool to structure photosensitive resists and create molds on which elastomeric solutions (PDMS) can be polymerized. Then we show that the structured PDMS can be specifically functionalized with biomolecules using UV-light structured by PRIMO and a specific photo-initiator (PLPP™). Altogether, we demonstrate that PRIMO allows to tailor the cell microenvironment topography through microfabrication and biochemistry through micropatterning.
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Anti-idiotypic binders for Trastuzumab validated in regulatory bioanalysis assay in collaboration with Covance
www.avacta.com/ >
Regulatory bioanalysis of therapeutic proteins during drug development and clinical follow-up requires the use of critical reagents that can specifically identify and accurately quantify each biotherapeutic within patient samples. Antibodies currently represent the 'gold standard' of affinity reagents used in the regulated bioanalysis of therapeutic proteins. While traditional antibodies have been refined to the point where they are specific, sensitive and reasonably reliable, they can be limited by their development speed, complexity to produce on an industrial scale and lot-to-lot variation in assay performance.
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Reducing sample preparation time from Sf9 insect cultures by using Sartoclear Dynamics® Lab
www.sartorius.com/sartoclear-dynamics-lab >
In this study, I tested the Sartoclear Dynamics® Lab V500 Kit as a novel method for the clarification of cell culture media prior to purification of a recombinant protein expressed in Sf9 cells. The vector from which this protein is expressed encodes a signal sequence to ensure secretion of the protein from the cells following expression. It is therefore necessary to remove cells from the media before purifying the protein but using traditional methods to do this (centrifugation followed by filtration) can be time consuming. Here, I show that the Sartoclear Dynamics® Lab filter offers significant time savings over conventional methods with no noticeable effect on protein yield, making this product an ideal addition to any laboratory looking to increase productivity and throughput from their insect cell expression systems.
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Miniaturized 16S rRNA Amplicon Sequencing with the Labcyte Echo® 525 Liquid Handler for Metagenomic and Microbiome Studies
www.labcyte.com >
Our understanding of the microbial communities in human health, metagenomics, and metataxonomics has been growing rapidly in recent years and is being increasingly elucidated every day. Amplicon sequencing of highly conserved prokaryotic 16S ribosomal RNA (rRNA) regions has long been the standard technique used to assess the diversity and phylogenetic classification of these communities. While advances in next-generation sequencing are enabling routine whole-genome shotgun sequencing in microbial communities, 16S rRNA amplicon sequencing is still frequently used for quick diagnosis of samples. Here, we perform a standard Illumina 16S rRNA amplicon sequencing library preparation at miniaturized scale using the Labcyte Echo® 525 Liquid Handler, effectively reducing reaction volumes and input sample while maintaining sufficient read depth to accurately capture the community. The result is a significantly cost-reduced workflow per sample, saving reagent costs, operational time costs, and valuable sample DNA.
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Improving biosensor assay development by determining sample quality with Tycho NT.6
nanotempertech.com/ >
Prime&Bond active is a one-component universal dental adhesive which brings new advantages to the universal adhesive arena. For eff ective bonding, conventional Universal Dental adhesives require ideal moisture conditions to form a homogeneous adhesive layer, and in turn a strong bond between tooth and restoration. However, when too much or too little moisture is present this may not happen and bonding can fail. Prime&Bond active contains "Active-Guard" technology, which gives this adhesive smart properties that compensate for variations in moisture levels and ensure complete adhesive coverage of the tooth.
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