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dinsdag 13 maart 2018

Nature Methods Application Notes e-UPDATE: 13 March 2018

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13 March 2018 
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Miniaturized 16S rRNA Amplicon Sequencing with the Labcyte Echo® 525 Liquid Handler for Metagenomic and Microbiome Studies
www.labcyte.com >
Our understanding of the microbial communities in human health, metagenomics, and metataxonomics has been growing rapidly in recent years and is being increasingly elucidated every day. Amplicon sequencing of highly conserved prokaryotic 16S ribosomal RNA (rRNA) regions has long been the standard technique used to assess the diversity and phylogenetic classification of these communities. While advances in next-generation sequencing are enabling routine whole-genome shotgun sequencing in microbial communities, 16S rRNA amplicon sequencing is still frequently used for quick diagnosis of samples. Here, we perform a standard Illumina 16S rRNA amplicon sequencing library preparation at miniaturized scale using the Labcyte Echo® 525 Liquid Handler, effectively reducing reaction volumes and input sample while maintaining sufficient read depth to accurately capture the community. The result is a significantly cost-reduced workflow per sample, saving reagent costs, operational time costs, and valuable sample DNA.

Making difficult things easier and simpler with a manipulation system
www.nsk.com >
Manipulators are used to perform fine operations under a microscopic field of view, and generally require a skilled technique. To create an easy-to-use manipulation system, we applied mechatronics and attached it to a microscope. Except for the replacement of the microscope's objective lens, this newly developed system is capable of all electric operation.

Rapid and real-time analysis of polyelectrolyte multilayer films using SEEC
www.nano-lane.com >
Polyelectrolyte multilayers offer novel biosynthetic interfaces that aid in high impact scientific advances in fields such as bioengineering. Current biosensor techniques employed to study the engineered biosynthetic surfaces do not offer real-time visualisation of the layer build-up process and/or surface characterisation studies such as topographic/morphologic analyses. SEEC technology offers enhanced surface contrast that permits quantification of nanometric changes on surfaces such as thin films in liquid conditions. We show that the newly automated N-Lab Station based on SEEC technology offers robust data reproducibility and comprehensive studies including surface characterisation of polyelectrolyte multilayers.

Hardware triggering: maximizing speed and efficiency for live cell imaging
www.nikoninstruments.com/Products/Inverted-Microscopes/Eclipse-Ti2 >
Live cell imaging experiments now require higher speeds and more data throughput than ever before. Nikon Instruments has robust tools that enable hardware triggering of imaging devices in microscopy via direct signaling between hardware. This minimizes delays, synchronizes devices, and reduces the exposure of specimens to light. This Application Note explains how Nikon's NIS-Elements hardware-triggering workflow operates, and details its benefits for common time-lapse acquisition routines.

Application of patterned illumination using a DMD for optogenetic control of signaling
www.nikoninstruments.com/Products/Photostimulation-and-TIRF/Ti-LAPP >
Digital micromirror devices (DMDs) are powerful tools for photostimulation applications, including photoconversion and optogenetic manipulation, owing to their robust ability to produce novel illumination patterns with high spatiotemporal resolution. In this Application Note we showcase recent work describing how DMD technology integrated into a Nikon system can be applied toward light-gated optogenetic control of intracellular signaling.

Monitoring neurite morphology and synapse formation in primary neurons for neurotoxicity assessments and drug screening
www.thermofisher.com/hcs >
Synapse formation during nervous system development and degeneration in the pathogenesis of human neurological diseases are highly regulated processes. Subtle changes in the environment of the complex neuronal network may cause either breakdown or creation of synaptic connections. Drug discovery screening for neurological diseases and compound neurotoxicity evaluation would benefit from robust, automated, quantitative in vitro assays that monitor neuronal function.

The new 2D Superresolution mode for ZEISS Airyscan
www.zeiss.com/airyscan >
Utilizing a pinhole-plane imaging concept, Airyscan allows for simultaneous improvement in resolution and signal-to-noise by capitalizing on an innovative 32-channel GaAsP photomultiplier tube (PMT) array detector. Each detection channel functions as a very small pinhole to increase resolution while the overall detector design delivers better signal-to-noise than traditional GaAsP-based confocal systems. In the past, a stack of at least five z-slices had to be deconvolved to get usable images with an optical section thinner than 1 Airy unit. Now, the new 2D Superresolution mode for Airyscan delivers images with the thinnest optical section (0.2 Airy units) from a single image while maintaining the light collection efficiency of a much larger 1.25-Airy-unit pinhole.


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