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Laboratory Investigation - Table of Contents alert Volume 97 Issue 12

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Laboratory Investigation
TABLE OF CONTENTS

Volume 97, Issue 12 (December 2017)

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Inside the USCAP Journals
Research Articles
Technical Reports

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Inside the USCAP Journals

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Inside the USCAP Journals

 

2017 97: 1398-1399; 10.1038/labinvest.2017.122

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Research Articles

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HEPATIC AND PANCREATIC SYSTEMS

Augmenter of liver regeneration potentiates doxorubicin anticancer efficacy by reducing the expression of ABCB1 and ABCG2 in hepatocellular carcinoma

Overexpression of augmenter of liver regeneration (alr) in hepatocellular carcinoma(hcc)downregulates expression of the transporters Abcb1 and Abcg2, leading to retention of the anti-cancer drug doxorubicin within Hcc cells. Alr sensitizes Hcc cells to the chemotherapeutical drug by its inhibition of the Akt/snail signaling pathway, consequentlyinactivating Abc transporter expression.

Yuan-yuan Guo, Yuan Wu, Xiao-wei Jia and Wei An

2017 97: 1400-1411; advance online publication, August 21, 2017; 10.1038/labinvest.2017.72

Abstract | Full Text

Upregulation of the actin cytoskeleton via myocardin leads to increased expression of type 1 collagen

Hepatic stellate cells become activated and transform into myofibroblasts during liver fibrosis. Myocardin promotes this process through upregulation of actin isoforms and the actin cytoskeleton. This in turn stimulates actin dynamics and concomitant actin functions as well as fibrogenesis and the liver wound healing response.

Zengdun Shi and Don C Rockey

2017 97: 1412-1426; advance online publication, October 16, 2017; 10.1038/labinvest.2017.96

Abstract | Full Text

GENITOURINARY AND REPRODUCTIVE SYSTEMS

Deletion of Pkd1 in renal stromal cells causes defects in the renal stromal compartment and progressive cystogenesis in the kidney

This study found that disruption of pkd1 (polycystin-1) from renal stromal cells causes defects in the renal stromal compartment, including reduced extracellular matrix and vascularrarefaction. Furthermore, renal epithelia exhibited increased proliferation and apoptosis associated with progressive cystogenesis in modelling of adulthood human autosomal dominant polycystic kidney disease.

Xuguang Nie and Lois J Arend

2017 97: 1427-1438; advance online publication, September 11, 2017; 10.1038/labinvest.2017.97

Abstract | Full Text

Vascular endothelial growth factor-C ameliorates renal interstitial fibrosis through lymphangiogenesis in mouse unilateral ureteral obstruction

In this study, the authors investigated the effect of vascular endothelial growth factor-c (vegf-c) on lymphangiogenesis, inflammation and fibrosis in mouse kidneys using a unilateral ureteral obstruction model of interstitial inflammation and fibrosis. Continuous Vegf-c administration induceslymphangiogenesis and attenuates renal fibrosis. Enhancement of the Vegf-c/vegfr-3 signaling pathway might be a therapeutic option for renal fibrotic diseases.

Shoko Hasegawa, Toshiaki Nakano, Kumiko Torisu, Akihiro Tsuchimoto, Masahiro Eriguchi, Naoki Haruyama, Kosuke Masutani, Kazuhiko Tsuruya and Takanari Kitazono

2017 97: 1439-1452; advance online publication, October 30, 2017; 10.1038/labinvest.2017.77

Abstract | Full Text

Correlation between E-cadherin interactions, survivin expression, and apoptosis in MDCK and ts-Src MDCK cell culture models

In a soft extracellular matrix environment, MDCK cells resemble adult tissue: they become fully differentiated, survivin is down-regulated, E-cadherin trans-interactions are well-developed, and the cells are susceptible to stress-induced apoptosis. In MDCK cells stably transfected with temperature sensitive viral Src, survivin is up-regulated and E-cadherin is relocated to the cytoplasm. Reactive oxygen species induces survivin down-regulation and apoptosis. The antioxidant N-acetylcysteine rescues the cells by returning E-cadherin to membranes.

Janne Capra and Sinikka Eskelinen

2017 97: 1453-1470; advance online publication, September 11, 2017; 10.1038/labinvest.2017.89

Abstract | Full Text

BLOOD, LYMPHATICS, IMMUNE SYSTEM AND STEM CELLS

Ligand-mediated cytoplasmic retention of the Ah receptor inhibits macrophage-mediated acute inflammatory responses

Two in vivo macrophage-driven acute mouse inflammation models were utilized to test whether the selective Ah receptor modulator SGA360 would reduce inflammation. Exposure to SGA360 is capable of significantly inhibiting lipopolysaccharide-mediated endotoxic shock and joint edema in a monosodium urate crystal gout models in an Ah receptor-dependent fashion.

Gulsum E Muku, Tejas S Lahoti, Iain A Murray, Michael A Podolsky, Kayla J Smith, Troy D Hubbard, Guray Kuzu, Krishne Gowda, Shantu G Amin and Gary H Perdew

2017 97: 1471-1487; advance online publication, September 11, 2017; 10.1038/labinvest.2017.92

Abstract | Full Text

ENDOCRINE, VISUAL AND AUDITORY SYSTEMS

Expression, function, and regulation of the embryonic transcription factor TBX1 in parathyroid tumors

The embryonic transcription factor TBX1, the candidate gene for 22q11.2-DiGeorge syndrome, which includes congenital hypoparathyroidism, is expressed in a subset of adult normal parathyroid glands and variably lost in parathyroid tumors as well as in fetal parathyroid glands. The authors show that TBX1 silencing is associated with cell cycle arrest and reduction of WNT5A; parathyroid tumors display cell heterogeneity and embryonic features.

Chiara Verdelli, Laura Avagliano, Vito Guarnieri, Filomena Cetani, Stefano Ferrero, Leonardo Vicentini, Edoardo Beretta, Alfredo Scillitani, Pasquale Creo, Gaetano Pietro Bulfamante, Valentina Vaira and Sabrina Corbetta

2017 97: 1488-1499; advance online publication, September 18, 2017; 10.1038/labinvest.2017.88

Abstract | Full Text

MODELS AND TECHNIQUES

A simple method based on Sanger sequencing and MS Word wildcard searching to identify Cas9-induced frameshift mutations

The authors developed a simple strategy to predict Crispr/cas9 induced indels, by analysis of the sequencing paragraph with wildcard searching (sws). This strategy reliably predicts if indels exist in each allele of the diploid genome and their exact numbers, which significantly facilitates the isolation of single clones harboring biallelic frame-shift alleles.

Hui Jie, Zhuoling Li, Ping Wang, Linjie Zhao, Qian Zhang, Xiaomin Yao, Xiangrong Song, Yinglan Zhao and Shaohua Yao

2017 97: 1500-1507; advance online publication, August 21, 2017; 10.1038/labinvest.2017.83

Abstract | Full Text

Technical Reports

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Whole tumor section quantitative image analysis maximizes between-pathologists’ reproducibility for clinical immunohistochemistry-based biomarkers

It is challenging for pathologists' quantitative scoring of immunohistochemistry-stained slides to meet high between-pathologist reproducibility. Technologies such as computational image analysis are available; however, its adoption is limited clinically. To increase image analysis's value proposition, the authors instituted a novel whole tumor section annotation approach across multiple modalities and biomarkers. This approach showed superiority compared to other modalities.

Michael Barnes, Chukka Srinivas, Isaac Bai, Judith Frederick, Wendy Liu, Anindya Sarkar, Xiuzhong Wang, Yao Nie, Bryce Portier, Monesh Kapadia, Olcay Sertel, Elizabeth Little, Bikash Sabata and Jim Ranger-Moore

2017 97: 1508-1515; advance online publication, August 14, 2017; 10.1038/labinvest.2017.82

Abstract | Full Text

Lung tumor exome files with T-cell receptor recombinations: a mouse model of T-cell infiltrates reflecting mutation burdens

It is challenging for pathologists' quantitative scoring of immunohistochemistry-stained slides to meet high between-pathologist reproducibility. Technologies such as computational image analysis are available; however, its adoption is limited clinically. To increase image analysis's value proposition, the authors instituted a novel whole tumor section annotation approach across multiple modalities and biomarkers. This approach showed superiority compared to other modalities.

Yaping N Tu, Wei Lue Tong, Timothy J Fawcett and George Blanck

2017 97: 1516-1520; advance online publication, August 14, 2017; 10.1038/labinvest.2017.80

Abstract | Full Text

High concordance of a closed-system, RT-qPCR breast cancer assay for HER2 mRNA, compared to clinically determined immunohistochemistry, fluorescence in situ hybridization, and quantitative immunofluorescence

Both immunohistochemistry and fluorescence in situ hybridization can be used to assess HER2 status, but these tests are time-consuming, expensive and they are sometimes equivocal. In this pilot study, the authors describe an inexpensive, rapid, closed system method for quantitative reverse transcription polymerase chain reaction assessment of HER2 that is highly concordant with current methods.

Brad E Wasserman, Daniel E Carvajal-Hausdorf, Kenneth Ho, Wendy Wong, Natalie Wu, Victor C Chu, Edwin W Lai, Jodi M Weidler, Michael Bates, Veronique Neumeister and David L Rimm

2017 97: 1521-1526; advance online publication, September 11, 2017; 10.1038/labinvest.2017.93

Abstract | Full Text

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