We bring you the latest news from the healthcare about the health care in the United Kingdom. Do your have news for us? Contact the editor. Watch also this special movie.

donderdag 30 november 2017

Nature Protocols Contents: Volume 12 Number 12, pp 2447-2659

If you are unable to see the message below, click here to view.
Nature Protocols

Advertisement
 

See what your cells are doing and when they do it with the IncuCyte® S3 Live-Cell Analysis System 

Biological processes are dynamic, and a single snapshot in time may not capture rare or transient events, causing you to miss a relevant response. With the IncuCyte® S3 live-cell analysis system, automatically follow the sequence of biological events continuously, then 'rewind and replay' the experiment to see what really happened to your cells while you were away. Learn how.. 

 
 
TABLE OF CONTENTS


December 2017, Volume 12 No 12
In this issue
Protocol Extension
Protocols
Advertisement


Analyze Large and Small Molecule Inhibitors in a Single Homogeneous Assay

Want to analyze downstream cell signaling events impacted by both large and small molecule inhibitors in a single assay? Learn about a no-wash platform for screening antibodies and small molecule inhibitors, in this example used to characterize chemokine receptor inhibitors. 

Download Application Note


Subscribe
 
Facebook
 
RSS
 
Recommend to library
 
Twitter
 
Advertisement
npj Quantum Materials is a new open access journal that is now open for submissions. The journal publishes broad coverage of quantum materials, their fundamental properties, fabrication and applications.

Sign up for article e-alerts >>>
 
PROTOCOL EXTENSION

Top

Identification of RNA-binding domains of RNA-binding proteins in cultured cells on a system-wide scale with RBDmap pp2447 - 2464
Here the authors provide an extension to their earlier RNA interactome capture protocol. This Protocol Extension describes RBDmap[mdash]a method to identify the regions of RNA-binding proteins engaged in native interactions with RNA, in a proteome-wide manner.
Alfredo Castello et al.
Published online: 02 November 2017 | doi:10.1038/nprot.2017.106
Abstract | Full Text | PDF (2,673K)

PROTOCOLS

Top

A high-throughput in vivo screening method in the mouse for identifying regulators of metastatic colonization pp2465 - 2477
In this protocol, the authors present an experimental metastasis assay in which cancer cells are injected into the tail vein of a mouse, and the resulting secondary organ colonization is assessed, primarily in the lung, 10 d later.
Anneliese O Speak et al.
Published online: 02 November 2017 | doi:10.1038/nprot.2017.118
Abstract | Full Text | PDF (2,540K)


Chromatin-state discovery and genome annotation with ChromHMM pp2478 - 2492
This protocol describes how to use ChromHMM, a robust open-source software package that enables the learning of chromatin states, annotates their occurrences across the genome, and facilitates their biological interpretation.
Jason Ernst and Manolis Kellis
Published online: 09 November 2017 | doi:10.1038/nprot.2017.124
Abstract | Full Text | PDF (1,644K)


Compartmentalized partnered replication for the directed evolution of genetic parts and circuits pp2493 - 2512
This protocol describes the procedures for compartmentalized partnered replication (CPR), an emulsion-based directed evolution method for the generation of proteins, genetic elements, and genetic circuits with improved or altered function.
Zhanar Abil et al.
Published online: 09 November 2017 | doi:10.1038/nprot.2017.119
Abstract | Full Text | PDF (1,639K)


Production of knock-in mice in a single generation from embryonic stem cells pp2513 - 2530
This protocol describes the generation of mice entirely derived from genome-edited embryonic stem cells, enabling the production of transgenic mice in a single generation.
Hideki Ukai, Hiroshi Kiyonari and Hiroki R Ueda
Published online: 16 November 2017 | doi:10.1038/nprot.2017.110
Abstract | Full Text | PDF (1,783K)


Multimodal profiling of single-cell morphology, electrophysiology, and gene expression using Patch-seq pp2531 - 2553
This protocol describes how to integrate whole-cell patch-clamp in single neurons from mouse brain tissue slices with single-cell RNA sequencing and morphological recovery.
Cathryn R Cadwell et al.
Published online: 16 November 2017 | doi:10.1038/nprot.2017.120
Abstract | Full Text | PDF (7,514K)


Chemical synthesis of membrane proteins by the removable backbone modification method pp2554 - 2569
This protocol describes how to chemically synthesize membrane proteins through the installation of solubilizing removable backbone modification tags into hydrophobic transmembrane peptides. The implementation of the protocol is demonstrated by the chemical synthesis of phosphorylated M2 (M2-pSer64), a 97-aa proton channel protein from the influenza A virus. The synthesis of M2-pSer64 at milligram scale takes [sim]200 working hours (excluding the time for lyophilizations).
Shan Tang et al.
Published online: 16 November 2017 | doi:10.1038/nprot.2017.129
Abstract | Full Text | PDF (1,381K)


Advertisement
Nature Collection: 2017 Nobel Prize in Chemistry 

Nature Research present this Collection of articles celebrating the 2017 Nobel Prize in Chemistry to Jacques Dubochet, Joachim Frank and Richard Henderson, recognised "for developing cryo-electron microscopy for the high-resolution structure determination of biomolecules in solution". 

Access the collection here >>

Produced with support from 
Thermo Fisher Scientific

Langmuir-Blodgett nanotemplates for protein crystallography pp2570 - 2589
Protein crystallization still presents a challenge for X-ray crystallography. This protocol describes the Langmuir-Blodgett nanotemplate method, in which 2D protein LB nanotemplates trigger formation of 3D protein crystals by hanging-drop vapor diffusion.
Eugenia Pechkova and Claudio Nicolini
Published online: 30 November 2017 | doi:10.1038/nprot.2017.108
Abstract | Full Text | PDF (7,516K)


Visualizing endocytic recycling and trafficking in live neurons by subdiffractional tracking of internalized molecules pp2590 - 2622
This protocol describes a pulse-chase approach to studying activity-dependent internalization of fluorescent ligands into endocytic compartments using subdiffractional single-particle tracking in live hippocampal neurons.
Merja Joensuu et al.
Published online: 30 November 2017 | doi:10.1038/nprot.2017.116
Abstract | Full Text | PDF (5,838K)


Preparation of viable adult ventricular myocardial slices from large and small mammals pp2623 - 2639
This protocol describes how to obtain 100- to 400-[mu]m-thick slices of a living myocardium from rodents, pigs, humans and dogs that retain the native multicellularity, architecture and physiology of the heart.
Samuel A Watson et al.
Published online: 30 November 2017 | doi:10.1038/nprot.2017.139
Abstract | Full Text | PDF (9,418K)


Deriving genotypes from RAD-seq short-read data using Stacks pp2640 - 2659
In this protocol, the authors provide a strategy and set of methods to analyze restriction-site-associated DNA-sequencing (RAD-seq) data using Stacks, enabling the genome-wide discovery and genotyping of SNPs across a range of systems.
Nicolas C Rochette and Julian M Catchen
Published online: 30 November 2017 | doi:10.1038/nprot.2017.123
Abstract | Full Text | PDF (1,503K)


Nature Protocols
JOBS of the week
Research Scientist in Epidemiology
Alberta Health Services, Cancer Epidemiology
Posdoctoral position
CNRS & Bordeaux University
Principal Scientist in Cellular Pharmacology
Johnson & Johnson
Junior and Senior Faculty Positions at the Department of Biology, SUSTech
Southern University of Science and Technology (SUSTech)
NIDDK / NIH Post-Doctoral Fellowship position
National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK)
More Science jobs from
Nature Protocols
EVENT


More science events from
nature events
Natureevents is a fully searchable, multi-disciplinary database designed to maximise exposure for events organisers. The contents of the Natureevents Directory are now live. The digital version is available here.

Find the latest scientific conferences, courses, meetings and symposia on natureevents.com. For event advertising opportunities across the Nature Publishing Group portfolio please contact natureevents@nature.com
More Nature Events

You have been sent this Table of Contents Alert because you have opted in to receive it. You can change or discontinue your e-mail alerts at any time, by modifying your preferences on your nature.com account at: www.nature.com/myaccount
(You will need to log in to be recognised as a nature.com registrant).

For further technical assistance, please contact our registration department

For print subscription enquiries, please contact our subscription department

For other enquiries, please contact our feedback department

Springer Nature | One New York Plaza, Suite 4500 | New York | NY 10004-1562 | USA

Springer Nature's worldwide offices:
London - Paris - Munich - New Delhi - Tokyo - Melbourne
San Diego - San Francisco - Washington - New York - Boston

Macmillan Publishers Limited is a company incorporated in England and Wales under company number 785998 and whose registered office is located at The Campus, 4 Crinan Street, London, N1 9XW.

© 2017 Macmillan Publishers Limited, part of Springer Nature. All Rights Reserved.

Springer Nature